37 CFR Nucleotide and/or amino acid sequence disclosures in patent applications. (a) Nucleotide and/or amino acid sequences as used in §§ through are interpreted to mean an unbranched sequence of four or more amino acids or an unbranched sequence of ten or more nucleotides. In molecular biology and genetics, the sense of a nucleic acid molecule, particularly of a strand of DNA or RNA, refers to the nature of the roles of the strand and its complement in specifying a sequence of amino datmelove.coming on the context, sense may have slightly different meanings. For example, DNA is positive-sense if an RNA version of the same sequence is translated or translatable.
Slightly expanded, this means that the deoxyribonucleic acidwhich is the genetic material in the nucleus of your cells, is used to make a similar molecule called RNA ribonucleic acid in a process called transcription. After this is done, RNA is used to direct the synthesis of proteins elsewhere in the cell in a process called translation.
Every organism is the sum of the proteins it makes, and in everything alive today and ever known to have lived, the information for strandd these proteins is stored in, and only in, that organism's DNA. Your DNA is what makes you what you are, and is what you pass on to any children you may have. In eukaryotic organisms, after the first step of transcription is complete, the newly synthesized messenger RNA mRNA must find its way outside the nucleus into the cytoplasm where translation takes place.
In prokaryotes, which lack nuclei, this is not the case. A,ino the plasma membrane surrounding the contents of the nucleus can be choosy, this process requires active input from the cell itself. Nucleic acids are macromolecules as they are composed of very long chains of repeating subunits, or monomers, called nucleotides.
Nucleotides themselves what is the amino acid sequence for the mrna strand of three distinct chemical components: sequenc five-carbon sugar, one to three phosphate groups, and one of four nitrogen-rich nitrogenous bases.
These sugars differ only in that ribose carries a hydroxyl -OH group attached to a carbon outside the five-membered ring where deoxyribose carries only a hydrogen atom -H. RNA has the first three, but includes uracil U in place of thymine. How to connect a dvd player to tv is double-stranded, with the two strands linked at their hhe bases. A always pairs with What is the amino acid sequence for the mrna strand, and C always pairs with G.
The sugar and phosphate groups create the backbone" of each so-called complementary strand. The resulting formation is a double helix, the shape of which was discovered in the s. The purpose of mRNA is to create a mobile, encoded set of directions for the synthesis of proteins. A length of DNA that includes the "blueprint" for a single protein product is called a gene.
Each three-nucleotide sequence carries the instructions for making a particular amino acid, with amino acids being the building blocks of proteins in the same way nucleotides are the building blocks of nucleic acids. There are 20 amino acids in all, allowing for an essentially limitless number of combinations and hence protein products. Transcription occurs in the nucleusalong a single strand of DNA that has become how to make a skylight in revit what is the amino acid sequence for the mrna strand its complementary strand for purposes of transcription.
After mRNA molecules are synthesized at the transcription site, they must make their journey to the sites of translation, the ribosomes. Ribosomes appear both free in the cell cytoplasm and attached to a membranous organelle called the endoplasmic reticulum, both of which lie outside the nucleus. Before the mRNA can pass through the double plasma membrane that makes up the nuclear envelope or nuclear membraneit must reach the membrane acic.
This occurs by the binding of the new how to defrost a frozen pipe molecules to ztrand proteins. Before the resulting mRNA-protein mRNP complexes can move to the edge, they become thoroughly mixed inside the substance of the nucleus, so that those mRNP complexes that happen to form near the edge of the nucleus have no better a chance at exiting the nucleus at a given time after formation than do mRNP processes close to the interior.
This stalling can be overcome by the input of energy in the form of ATP, which prods the bogged-down mRNP in the direction of the edge of the nucleus. The nucleus needs to protect the all-important genetic material of the cell, yet it also must have a means of exchanging proteins and nucleic acids with the cell cytoplasm. This is accomplished via "gates" consisting of proteins and known as nuclear pore complexes NPC. These complexes have a pore running through the double membrane of the nuclear envelope and amlno number of different structures on either side of this "gate.
The NPC is enormous by molecular standards. In human beings, it has a molecular mass of million Daltons. In contrast, a molecule of glucose has a molecular mass of Daltons, making it abouttimes smaller than the NPC complex. Both nucleic acid and protein transport into the nucleus and the movement of these molecules out of the nucleus occur via the NPC. On the cytoplasmic side, the NPC has what is called a cytoplasmic ring as well as cytoplasmic filaments, both of which serve to help anchor the NPC in place in the nuclear membrane.
On the nuclear side of the NPC is a nuclear ring, analogous to the cytoplasmic ring on the opposite side, as well as a nuclear basket. A variety of individual proteins participate in the movement of mRNA and a diverse variety of other molecular cargoes out of the nucleus, with the same applying to movement of substances into the nucleus. Each ribosome in the whwt or attached to endoplasmic reticulum consists of a large and a small subunit; these only come together when the ribosome is active in transcription.
When an mRNA molecule becomes attached to a translation site along the ribosome, it is joined by a particular kind of tRNA that carries a how to copy a cd disk amino acid there are therefore 20 different flavors of tRNA, one for each amino acid.
This occurs because the tRNA can "read" the three-nucleotide sequence on the exposed mRNA that seqyence to a given amino acid. This polypeptide reaches its specified length when the mRNA molecule is read in its entirety, and the polypeptide is released and processed into a bona fide protein. Kevin Beck holds a bachelor's degree in physics with minors in math and chemistry from the University of Vermont.
Formerly with ScienceBlogs. More about Kevin and links amnio his professional work can be found at www. Copyright Leaf Group Ltd.
May 31, · When an mRNA molecule becomes attached to a translation site along the ribosome, it is joined by a particular kind of tRNA that carries a specific amino acid (there are therefore 20 different flavors of tRNA, one for each amino acid). This occurs because the tRNA can "read" the three-nucleotide sequence on the exposed mRNA that corresponds to a. Nucleic acids are biopolymers, or large biomolecules, essential to all known forms of datmelove.com are composed of nucleotides, which are the monomers made of three components: a 5-carbon sugar, a phosphate group and a nitrogenous datmelove.com two main classes of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). If the sugar is ribose, the polymer is RNA; if the sugar is the.
These tables are reproduced below. Note that the standard was revised in December , and the current version is available online at www. The symbols from the list below may be used in the description i. Modifications not listed in Table 2 may also be represented as the corresponding unmodified base in the sequence itself, and the modification should be described using its full chemical name in the Feature section of the sequence listing.
Modifications not listed in Table 4 may also be represented as the corresponding unmodified amino acid in the sequence itself, and the modification should be described using its full chemical name in the Feature section of the sequence listing. The requirements of 37 CFR 1. PatentIn Version 3. Requirements related to the submission of sequence listings may also differ between filing in the United States and filing internationally.
For example, where an international application is filed in paper, the sequence listing part of the international application must also be provided in paper, although the search copy must be filed in electronic form, e. Also, any tables filed in an international application must be an integral part of the application, i.
Branched sequences are specifically excluded from this definition. Sequences with fewer than ten specifically defined nucleotides or four specifically defined amino acids are specifically excluded from this section. The limit of four or more amino acids was established for consistency with limits in place for industry database collections whereas the limit of ten or more nucleotides, while lower than certain industry database limits, was established to encompass those nucleotide sequences to which the smallest probe will bind in a stable manner.
Situations in which the applicability of the rules is in issue will be resolved on a case-by-case basis. Nucleotide sequences are further limited to those that can be represented by the symbols set forth in 37 CFR 1. The presence of other than typical 5' to 3' phosphodiester linkages in a nucleotide sequence does not render the rules inapplicable.
The Office does not want to exclude linkages of the type commonly found in naturally occurring nucleotides, e. Amino acid sequences are further limited to those listed in 37 CFR 1. The presence of one or more D-amino acids in a sequence will exclude that sequence from the scope of the rules. Voluntary compliance is, however, encouraged in these situations; the symbol "Xaa" can be used to represent D-amino acids.
With regard to amino acid sequences, the use of the terms "peptide or protein" implies, however, that the amino acids in a given sequence are linked by at least three consecutive peptide bonds. Accordingly, an amino acid sequence is not excluded from the scope of the rules merely due to the presence of a single non-peptidyl bond. If an amino acid sequence can be represented by a string of amino acid abbreviations, with reference, where necessary, to a features table to explain modifications in the sequence, the sequence comes within the scope of the rules.
However, the rules are not intended to encompass the subject matter that is generally referred to as synthetic resins. The requirement for compliance in 37 CFR 1. All sequence information, whether claimed or not, that meets the length thresholds in 37 CFR 1. The goal of the Office is to build a comprehensive database that can be used for, inter alia, assessing the prior art. It is therefore essential that all sequence information, whether only disclosed or also claimed, be included in the database.
However, if the applicant presents the sequence as a string of particular nucleotide bases or amino acids, it is necessary to include the sequence in the sequence listing regardless of whether the applicant considers the sequence to be prior art.
However, the primary sequence should be annotated in the sequence listing to reflect such variants. By way of example only, the following types of sequence disclosures would be treated as noted herein by the Office.
With respect to a primary sequence and "conservatively modified variants thereof," the sequences may be described as SEQ ID NO:X the primary sequence and "conservatively modified variants thereof," if desired. With respect to a sequence that "may be deleted at the C-terminus by 1, 2, 3, 4, or 5 residues," all of the implied variations do not need to be included in the sequence listing. In this latter example, only the sequence without deletions needs to be included in the sequence listing, however applicant is encouraged to annotate the sequence to indicate that deletions have been made at the C-terminus by 1, 2, 3, 4, or 5 residues.
The Office's database will only contain the unmodified sequence. It is strongly recommended that any sequences appearing in the claims, or sequences that are considered essential to understanding the invention, be included in the sequence listing as a separate sequence.
The requirement for sequence identifiers, at a minimum, requires that each sequence be assigned a different number for purposes of identification.
However, where practical and for ease of reference, sequences should be presented in the sequence listing in numerical order and in the order in which they are discussed in the application. This requirement is also intended to permit references elsewhere in the application e. For example, language such as "residues 14 to of SEQ ID NO" is permissible and the fragment need not be separately presented in the sequence listing.
Where a sequence that meets the length thresholds of 37 CFR 1. The rules do not alter, in any way, the requirements of 35 U. The rules, in general, or the use of sequence identifiers throughout the specification and claims, specifically, should not raise any issues under 35 U.
These identification numbers do not in any way restrict the manner in which an invention can be claimed. This requirement is necessary to minimize any confusion that could result if more than one format for representing sequence data was employed in a given application.
Pursuant to 37 CFR 1. However many significant sequence characteristics may only be demonstrated by a figure. This is especially true in view of the fact that the representation of double stranded nucleotides is not permitted in the sequence listing and many significant nucleotide features, such as "sticky ends" and the like, may only be shown effectively by reference to a drawing figure.
Similarly, drawing figures are recommended for use with amino acid sequences to depict structural features of the corresponding protein, such as finger regions and Kringle regions.
The situations discussed herein are given by way of example only and there may be many other reasons for including a sequence in a drawing. However, when a sequence is presented in a drawing, the sequence must still be included in the sequence listing if the sequence falls within the definition set forth in 37 CFR 1. This separate part of the disclosure is referred to as the sequence listing. The sequence listing required pursuant to 37 CFR 1.
The sequence listing required by 37 CFR 1. If submitted on paper, the sequence listing is a separate part of the disclosure which must begin on a new page within the specification. A plurality of sequences may, if feasible, be presented on a single page; the separate presentation of both nucleotide and amino acid sequences on the same page is also permitted. Note that 37 CFR 1.
The Office strongly suggests filing the sequence listing required by 37 CFR 1. If the official copy of the sequence listing as required by 37 CFR 1. The total number of compact discs including duplicates and the files on each compact disc shall be specified 37 CFR 1. The compact disc used to submit the sequence listing may also contain table information if the table has more than 50 pages of text. See 37 CFR 1. The compact disc and duplicate copy must be labeled "Copy 1" and "Copy 2," respectively, and a statement stating that the copies are identical must be included.
If the two compact discs are not identical, the Office will use the disc labeled "Copy 1" for further processing 37 CFR 1. If the sequence listing under 37 CFR 1. If the CRF is also submitted on compact disc, applicants will need to submit a total of three copies of the sequence listing one pursuant to 37 CFR 1. The sequence listing must be a single document, but the document may be split amongst two or more compact discs using software designed to divide a file that is too large to fit on a single compact disc into multiple concatenated files.
If the user breaks up a sequence listing so that it may be submitted on multiple compact discs, the compact discs must be labeled to indicate their order e.
One hundred megabytes is the size limit for sequence listing text files submitted via EFS-Web. If a user wishes to submit an electronic copy of a sequence listing text file that exceeds megabytes, the sequence listing must be filed on compact disc s. Effective for submissions filed on or after January 16, , the Office set two new fees to manage handling of sequence listings of MB or more in 37 CFR 1. The fee should encourage applicants to draft their specifications such that sequence data that is not essential material is not required to be included in a sequence listing.
A reduced number of mega-sequence listings will benefit the Office and the public by reducing the strain on Office resources, thus facilitating the effective administration of the patent system.
The fee under 37 CFR 1. As an example, if an application was filed prior to January 16, with or without a text file sequence listing , and thereafter a mega-sequence listing that is between and MB is filed, the fee under 37 CFR 1.
If an applicant thereafter files a corrected sequence listing that is also between and MB, no additional fee is due. If a further corrected sequence listing is filed and the file size exceeds MB, then the total fee owed under 37 CFR 1. The fee is due upon submission of the mega-sequence listing. Subsequent deletion or reduction in size of a sequence listing does not change the requirement to pay the mega-sequence listing submission fee. The information below is specific to sequence listing submissions via EFS-Web.
Thus, the following are not required and should not be submitted: 1 a second copy of the sequence listing in a PDF file; 2 a statement under 37 CFR 1. Checker software that may be used to check a sequence listing for compliance with the requirements of 37 CFR 1. The User Notes on the Checker website should be consulted for an explanation of errors that are not indicated, and content that is not verified, by the Checker software.
If a user submits a sequence listing under 37 CFR 1. In addition, if a user submits an amendment to, or a replacement of, a sequence listing under 37 CFR 1. An incorporation-by-reference statement of the sequence listings is also required in both of these instances.
Submission of the sequence listing in a PDF file on the application filing date is not recommended. Any sequence listing submitted in PDF format or on paper on the application filing date is treated as the paper copy required by 37 CFR 1.
Regarding a table submitted as an ASCII text file via EFS-Web that is part of the specification or drawings, each three kilobytes of content submitted will be counted as a sheet of paper for purposes of determining the application size fee required by 37 CFR 1. Each table should be submitted as a separate text file. Further, the file name for each table should indicate which table is contained therein.
See subsection IV, below, for additional information regarding application size fees in an international application PCT. If a user wishes to submit an electronic copy of a sequence listing text file that exceeds megabytes, it is recommended that the user file the application without the sequence listing using EFS-Web to obtain the application number and confirmation number, and then file the sequence listing on compact disc in accordance with 37 CFR 1.
Note: a submission of a sequence listing in electronic form of MB or more in size is subject to the fee set forth in 37 CFR 1. Alternatively, a user may submit the application on paper and include the electronic copy of the sequence listing text file on compact disc in accordance with 37 CFR 1. Sequence listing text files may not be partitioned into multiple files for filing via EFS-Web as the EFS-Web system is not currently capable of handling such submissions.
If the sequence listing is filed on a compact disc, the sequence listing must be a single document, but the document may be split for submission on multiple physical media using software designed to divide a file into multiple files for subsequent concatenation. See subsection IV. B, below, for information regarding submission of a sequence listing text file that exceeds megabytes in an international application PCT filed via EFS-Web.